Description
Intended Uses: The Herpes simplex Virus 2 (HSV 2) IgG ELISA is intended for the determination of IgG class antibodies against Herpes simplex Virus 2 (HSV 2) in human serum or plasma (citrate, heparin). For research use only -
Principle of the Assay: The immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Im-munosorbent Assay) technique. Microtiterplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound con-jugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction prod-uct. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sul-phuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA Microtiterplate reader